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Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Co
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CrimsonPhantom Offline
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Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Co
Quote:The Wuhan Institute of Virology assembled a monkeypox virus genome, allowing the virus to be identified through PCR tests, using a method researchers flagged for potentially creating a “contagious pathogen,” The National Pulse can reveal.

The study was first published in February 2022, just months before the latest international outbreak of monkeypox cases which appear to have now reached the United States.

The paper, which was authored by nine Wuhan Institute of Virology researchers and published in the lab’s quarterly scientific journal Virologica Sinica, also follows the wide-scale use of Polymerase Chain Reaction (PCR) tests to identify COVID-19-positive individuals.

Researchers appeared to identify a portion of the monkeypox virus genome, enabling PCR tests to identify the virus, in the paper: “Efficient Assembly of a Large Fragment of Monkeypox Virus Genome as a qPCR Template Using Dual-Selection Based Transformation-Associated Recombination.”

Monkey pox viruses – referred to as “MPXVs” in the paper – have strains that are “more pathogenic and [have] been reported to infect humans in various parts of the world.”

“Quantitative polymerase chain reaction (qPCR) is the gold standard for the detection of orthopoxvirus (including MPXV). For pan-orthopoxviruses detection, the E9L (DNA polymerase) gene has been shown to be an excellent target for qPCR assays. For MPXV detection, Li et al. reported that the C3L (complement-binding protein) gene could be used as the qPCR target for the MPXV Congo Basin strain,” explained the paper before noting that China lacked sufficient genetic information on the virus for PCR detection:

“Since MPXV infection has never been associated with an outbreak in China, the viral genomic material required for qPCR detection is unavailable. In this report, we employed dual-selective TAR to assemble a 55-kb MPXV genomic fragment that encompasses E9L and C3L, two valuable qPCR targets for detecting MPXV or other orthopoxviruses.”

“The primary purpose of assembling a fragment of the MPXV genome is to provide a nucleotide template for MPXV detection,” reiterated the study, which relied on the process of transformation-associated recombination (TAR) to isolate a genomic fragment of the monkeypox virus.

“As an efficient tool for assembling large DNA fragments up to 592 kb in length, TAR assembly has become essential for preparing infectious clones of large DNA/RNA viruses,” explained researchers.

The paper acknowledged that TAR “applied in virological research could also raise potential security concerns, especially when the assembled product contains a full set of genetic material that can be recovered into a contagious pathogen.”

“In this study, although a full-length viral genome would be the ideal reference template for detecting MPXV by qPCR, we only sought to assemble a 55-kb viral fragment, less than one-third of the MPXV genome. This assembly product is fail-safe by virtually eliminating any risk of recovering into an infectious virus while providing multiple qPCR targets for detecting MPXV or other Orthopoxviruses,” posited researchers.

The unearthed study follows the Wuhan Institue of Virology conducting similar research into strains of bat coronaviruses that could infect humans while admitting its facilities lacked proper laboratory safety protocols.

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05-22-2022 02:18 PM
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maximus Offline
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Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Co
Everyone involved with that gosh damn place should be executed

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(This post was last modified: 05-22-2022 02:27 PM by maximus.)
05-22-2022 02:27 PM
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Eagleaidaholic Offline
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RE: Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Co
And I'm sure we paid for it. Pretty obvious The "Wuhan Lab" is a viral weapon lab and we give them money. Geniuses.
05-22-2022 02:38 PM
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GoodOwl Offline
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RE: Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Co
The last monkeypox outbreak in the U.S. was in 2003 when 47 people had confirmed or probable cases. The illness was first identified by scientists in 1958 when there were two outbreaks of a “pox-like” disease in research monkeys — thus the name monkeypox. It is estimate there are thousands of monkeypox infections in about a dozen African countries every year. Most are in Congo, which reports about 6,000 cases annually, and Nigeria, with about 3,000 cases a year. The first known human infection was in 1970, in a 9-year-old boy in Africa – where the disease is now endemic.

Isolated cases of monkeypox are occasionally spotted outside Africa, including in the U.S. and Britain. The cases are typically associated with travel to Africa or contact with animals from areas where the disease is more common. Apparently there was some kind of gay fetish festival this year in Belgium, and, whaddayaknow, one of the "participants" had it already and spread it to other humans. So it appears a takeaway lesson is: don't go participate in gay festivals if you don't want to be at risk for monkeypox. But somehow, it seems likely the MSM narrative will seek to avoid this inconvenient fact.

edit: Also don't seem to remember any widespread news coverage of the 2003 US outbreak of monkeypox, nor the admonition from crazed "health officials" that everyone needs to 'mask up' and lockdowns would be 'necessary' along with thousands of un-monitored voting dropboxes 'needing' to be immediately made available, along with hundreds of millions of dollars of vaccines needing to be purchased by governments and given to everyone quickly without argument or debate. Funny how the US (and the rest of the world) somehow "survived" THAT outbreak of monkeypox, but of course our "leaders" now "know better", right?
(This post was last modified: 05-22-2022 07:18 PM by GoodOwl.)
05-22-2022 07:12 PM
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CardinalJim Offline
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RE: Wuhan Lab Recently Assembled Monkeypox Strains Using Methods Flagged For Creating ‘Co
(05-22-2022 02:38 PM)Eagleaidaholic Wrote:  And I'm sure we paid for it. Pretty obvious The "Wuhan Lab" is a viral weapon lab and we give them money. Geniuses.

A viral weapon for the Far Left to help push their communist agenda. China is more than happy to work hand in hand with the communists in the Democratic Party.
05-22-2022 07:22 PM
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