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(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  

(03-18-2020 04:42 PM)Bearcatbdub Wrote:  

(03-18-2020 01:53 PM)converrl Wrote:  

(03-17-2020 10:38 PM)Bearcatbdub Wrote:  Zinc never got a P value worth a damn. By all means take it if it makes you feel better (most likely placebo effect), but I don’t recommend it in high doses.

https://www.mayoclinic.org/diseases-cond...q-20057769

Well:

There is this:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273967/

Better statistics. It may lessen cold symptoms by a day. And it may decrease the amount of colds by 1/2 a cold per season (whatever that means everytime I get a cold it’s a whole one) if taken as daily prophylaxis. However, this is at the expense of a bad taste in your mouth and potentially nausea. Not to mention it interferes with copper metabolism in the body and long term use has never been studied. You have to ask yourself if the side effects are worth it- that’s on the individual.

So like I said, if it helps you by all means have at it. As a practicing pharmacist of 20 years- I wouldn’t recommend it to everybody.

I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

You don't harvest it from plasma for distribution. You sequence the antibody that lights up on the ELISA and then synthesize it the same way they make peptides for research. The other option would be to produce it from monoclonals and harvest them from tissue culture. This is how the major biotech firms produce monoclonal for research.

They just announced the chloroquine results. Told you zinc was part of the answer.

(03-19-2020 07:43 AM)converrl Wrote:  

(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  

(03-18-2020 04:42 PM)Bearcatbdub Wrote:  

(03-18-2020 01:53 PM)converrl Wrote:  Well:

There is this:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273967/

Better statistics. It may lessen cold symptoms by a day. And it may decrease the amount of colds by 1/2 a cold per season (whatever that means everytime I get a cold it’s a whole one) if taken as daily prophylaxis. However, this is at the expense of a bad taste in your mouth and potentially nausea. Not to mention it interferes with copper metabolism in the body and long term use has never been studied. You have to ask yourself if the side effects are worth it- that’s on the individual.

So like I said, if it helps you by all means have at it. As a practicing pharmacist of 20 years- I wouldn’t recommend it to everybody.

I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

You don't harvest it from plasma for distribution. You sequence the antibody that lights up on the ELISA and then synthesize it the same way they make peptides for research. The other option would be to produce it from monoclonals and harvest them from tissue culture. This is how the major biotech firms produce monoclonal for research.

They just announced the chloroquine results. Told you zinc was part of the answer.

(03-19-2020 01:54 PM)Captain Bearcat Wrote:  

(03-19-2020 07:43 AM)converrl Wrote:  

(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  

(03-18-2020 04:42 PM)Bearcatbdub Wrote:  Better statistics. It may lessen cold symptoms by a day. And it may decrease the amount of colds by 1/2 a cold per season (whatever that means everytime I get a cold it’s a whole one) if taken as daily prophylaxis. However, this is at the expense of a bad taste in your mouth and potentially nausea. Not to mention it interferes with copper metabolism in the body and long term use has never been studied. You have to ask yourself if the side effects are worth it- that’s on the individual.

So like I said, if it helps you by all means have at it. As a practicing pharmacist of 20 years- I wouldn’t recommend it to everybody.

I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

You don't harvest it from plasma for distribution. You sequence the antibody that lights up on the ELISA and then synthesize it the same way they make peptides for research. The other option would be to produce it from monoclonals and harvest them from tissue culture. This is how the major biotech firms produce monoclonal for research.

They just announced the chloroquine results. Told you zinc was part of the answer.

No doubt!

...and here comes the antibody I was talking about...

https://www.foxnews.com/media/dr-jacob-g...oronavirus

...another story about chloroquine and the use of antibody from recovered patients...

https://www.fda.gov/news-events/press-an...treatments

(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  

(03-18-2020 04:42 PM)Bearcatbdub Wrote:  

(03-18-2020 01:53 PM)converrl Wrote:  

(03-17-2020 10:38 PM)Bearcatbdub Wrote:  Zinc never got a P value worth a damn. By all means take it if it makes you feel better (most likely placebo effect), but I don’t recommend it in high doses.

https://www.mayoclinic.org/diseases-cond...q-20057769

Well:

There is this:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273967/

Better statistics. It may lessen cold symptoms by a day. And it may decrease the amount of colds by 1/2 a cold per season (whatever that means everytime I get a cold it’s a whole one) if taken as daily prophylaxis. However, this is at the expense of a bad taste in your mouth and potentially nausea. Not to mention it interferes with copper metabolism in the body and long term use has never been studied. You have to ask yourself if the side effects are worth it- that’s on the individual.

So like I said, if it helps you by all means have at it. As a practicing pharmacist of 20 years- I wouldn’t recommend it to everybody.

I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

Honestly, who wouldn't advise giving Renee Russo a hot injection?

(03-19-2020 07:43 AM)converrl Wrote:  

(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  

(03-18-2020 04:42 PM)Bearcatbdub Wrote:  

(03-18-2020 01:53 PM)converrl Wrote:  Well:

There is this:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273967/

Better statistics. It may lessen cold symptoms by a day. And it may decrease the amount of colds by 1/2 a cold per season (whatever that means everytime I get a cold it’s a whole one) if taken as daily prophylaxis. However, this is at the expense of a bad taste in your mouth and potentially nausea. Not to mention it interferes with copper metabolism in the body and long term use has never been studied. You have to ask yourself if the side effects are worth it- that’s on the individual.

So like I said, if it helps you by all means have at it. As a practicing pharmacist of 20 years- I wouldn’t recommend it to everybody.

I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

You don't harvest it from plasma for distribution. You sequence the antibody that lights up on the ELISA and then synthesize it the same way they make peptides for research. The other option would be to produce it from monoclonals and harvest them from tissue culture. This is how the major biotech firms produce monoclonal for research.

They just announced the chloroquine results. Told you zinc was part of the answer.

There's nothing quite like seeking medical advice from a sports chatroom, so here's my suggestion: To get a therapeutic dose of zinc, lick an old galvanized bucket.

(03-21-2020 11:41 AM)colohank Wrote:  

(03-19-2020 07:43 AM)converrl Wrote:  

(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  

(03-18-2020 04:42 PM)Bearcatbdub Wrote:  Better statistics. It may lessen cold symptoms by a day. And it may decrease the amount of colds by 1/2 a cold per season (whatever that means everytime I get a cold it’s a whole one) if taken as daily prophylaxis. However, this is at the expense of a bad taste in your mouth and potentially nausea. Not to mention it interferes with copper metabolism in the body and long term use has never been studied. You have to ask yourself if the side effects are worth it- that’s on the individual.

So like I said, if it helps you by all means have at it. As a practicing pharmacist of 20 years- I wouldn’t recommend it to everybody.

I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

You don't harvest it from plasma for distribution. You sequence the antibody that lights up on the ELISA and then synthesize it the same way they make peptides for research. The other option would be to produce it from monoclonals and harvest them from tissue culture. This is how the major biotech firms produce monoclonal for research.

They just announced the chloroquine results. Told you zinc was part of the answer.

There's nothing quite like seeking medical advice from a sports chatroom, so here's my suggestion: To get a therapeutic dose of zinc, lick an old galvanized bucket.

Again...you have to consider the source... my perspective comes from 25 years in research as a molecular biologist...during which time I worked with...you guessed it....viruses.

His perspective is as a pharmacist....I'd pay attention to both of us.

(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  

(03-18-2020 04:42 PM)Bearcatbdub Wrote:  

(03-18-2020 01:53 PM)converrl Wrote:  

(03-17-2020 10:38 PM)Bearcatbdub Wrote:  Zinc never got a P value worth a damn. By all means take it if it makes you feel better (most likely placebo effect), but I don’t recommend it in high doses.

https://www.mayoclinic.org/diseases-cond...q-20057769

Well:

There is this:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273967/

Better statistics. It may lessen cold symptoms by a day. And it may decrease the amount of colds by 1/2 a cold per season (whatever that means everytime I get a cold it’s a whole one) if taken as daily prophylaxis. However, this is at the expense of a bad taste in your mouth and potentially nausea. Not to mention it interferes with copper metabolism in the body and long term use has never been studied. You have to ask yourself if the side effects are worth it- that’s on the individual.

So like I said, if it helps you by all means have at it. As a practicing pharmacist of 20 years- I wouldn’t recommend it to everybody.

I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

(03-21-2020 12:01 PM)converrl Wrote:  

(03-21-2020 11:41 AM)colohank Wrote:  

(03-19-2020 07:43 AM)converrl Wrote:  

(03-18-2020 10:22 PM)Bearcatbdub Wrote:  

(03-18-2020 08:54 PM)converrl Wrote:  I use Zicam and have never had a problem with the taste. I've also used daily supplements. Generally I'll double up with the lozenges and the nasal swabs with the 1st hint of a sore throat and it's always done fine by me.

I really like the RNA data...seems this is one of the few anti-virals that goes after a unique process in the virus (RNA dependent RNA synthesis) same way that AZT hits retroviruses at a unique target. Combining it with choroquine jacks up the intracellular zinc levels making it even more effective.

What I'd really like to see is purification of antibody from cases that have shown recovery...test for binding by ELISA and then mass-produce and market that as a passive immunization while an active vaccine is developed...always thought that would be a sound approach.

I am not a bio engineer, but that last bit of dipsy doo sounds like it would take forever. Not sure how your going to pool enough plasma from survivors to get that done in amounts that would be clinically feasible for treatment. We have a hard enough time getting the garden variety of IgG let alone what you are talking about. Plus, we aren’t even talking about clinical trials for safety let alone efficacy, that can’t just be done overnight like Dustin Hoffman grabbing the monkey serum in Outbreak and giving a hot injection to Renee Russo.

You don't harvest it from plasma for distribution. You sequence the antibody that lights up on the ELISA and then synthesize it the same way they make peptides for research. The other option would be to produce it from monoclonals and harvest them from tissue culture. This is how the major biotech firms produce monoclonal for research.

They just announced the chloroquine results. Told you zinc was part of the answer.

There's nothing quite like seeking medical advice from a sports chatroom, so here's my suggestion: To get a therapeutic dose of zinc, lick an old galvanized bucket.

Again...you have to consider the source... my perspective comes from 25 years in research as a molecular biologist...during which time I worked with...you guessed it....viruses.

His perspective is as a pharmacist....I'd pay attention to both of us.

When you started talking about ELISA, I know you were playing with a loaded deck.