https://www.youtube.com/watch?v=MneMC5W1fZ0

This young man did a great job. I don't think actual expansion plans are made based on the results of data analysis. Rather, I believe it's almost entirely about money but - still - great job.

To get to the alignment aspect: This project resulted in the MLB expanding to 32 with Raleigh, North Carolina and San Jose, California. If that were the case, how would MLB align?

I would guess the league would do its best to put 1 new franchise in each the AL and NL and avoid shifting teams between the AL and NL. A 2x4x4 setup is where I landed.

American League
Central: Houston Astros, Kansas City Royals, Minnesota Twins, Texas Rangers
East: Baltimore Orioles, Boston Red Sox, New York Yankees, Tampa Bay Rays
North: Chicago White Sox, Cleveland Guardians, Detroit Tigers, Toronto Blue Jays
West: Las Vegas Athletics, Los Angeles Angels, San Jose Expansion, Seattle Mariners

National League
Central: Chicago Cubs, Colorado Rockies, Milwaukee Brewers, St. Louis Cardinals
East: Atlanta Braves, Miami Marlins, Raleigh Expansion, Washington Nationals
North: Cincinnati Reds, New York Mets, Philadelphia Phillies, Pittsburgh Pirates
West: Arizona Diamondbacks, Los Angeles Dodgers, San Diego Padres, San Francisco Giants

My preference would be to realign geographically:

Eastern Conference
Atlantic: Baltimore Orioles, Pittsburgh Pirates, Toronto Blue Jays, Washington Nationals
Mideast: Chicago White Sox, Cincinnati Reds, Cleveland Guardians, Detroit Tigers
Northeast: Boston Red Sox, New York Mets, New York Yankees, Philadelphia Phillies
Southeast: Atlanta Braves, Miami Marlins, Raleigh Expansion, Tampa Bay Rays

Western Conference
Central: Colorado Rockies, Houston Astros, Kansas City Royals, Texas Rangers
Midwest: Chicago Cubs, Milwaukee Brewers, Minnesota Twins, St. Louis Cardinals
Pacific: Las Vegas Athletics, San Francisco Giants, San Jose Expansion, Seattle Mariners
Southwest: Arizona Diamondbacks, Los Angeles Angels, Los Angeles Dodgers, San Diego Padres

San Jose is not happening due to the Giants. The state of Utah has committed $900 million of public money for MLB, that is going to be hard for MLB to pass up.

I don't think we'll see radical realignment because the Chicago teams would be the natural division point between the two conferences. Even if MLB expands with two teams west of Chicago, that would then split Saint Louis and the Cubs. The Cubs also likely would not want to be in the western conference regardless.

The only bad part about expanding with one team on the West and one team in the East is that this would give MLB nine teams in the Mountain and Pacific Time Zones, which doesn't work well with divisions of four teams. The only exception would be the western expansion team being awarded to Austin:

AL West: Seattle, Athletics, Angels, Colorado
AL Central: Texas, Houston, Kansas City, Minnesota
AL North: White Sox, Cleveland, Detroit, Toronto
AL East: Boston, Yankees, Baltimore, Tampa Bay

NL West: San Francisco, Dodgers, San Diego, Arizona
NL Central: Austin, Saint Louis, Milwaukee, Cubs
NL South: Raleigh, Atlanta, Washington, Miami
NL East: Mets, Philadelphia, Pittsburgh, Cincinnati

Realigning AAA would be a bit of an issue as the 10 teams in the Pacific Coast League mirror the 10 teams in the current AL West and NL West. In this scenario the PCL would drop to eight teams with a third AAA league being formed to mirror the AL and NL Central. This would require the Dodgers to move their affiliation out of Oklahoma City.

PCL: Fresno (from Oklahoma City), Tacoma, Sacramento, Reno, Salt Lake, Las Vegas, El Paso, Albuquerque

New League: Round Rock, Sugar Land, Iowa, Saint Paul, Omaha, Memphis, Wichita (new), Oklahoma City (new)

IL: Indianapolis, Louisville, Nashville, Gwinnett, Jacksonville, Charlotte, Durham, Norfolk, Toledo, Columbus, Lehigh Valley, Scranton, Buffalo, Rochester, Syracuse, Worcester

The Texas League team in Wichita should have plenty of relocation options in the Texas League footprint.